Functional expression of a human Na+/H+ antiporter gene transfected into antiporter-deficient mouse L cells.
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چکیده
منابع مشابه
Functional expression of a human Na+/H+ antiporter gene transfected into antiporter-deficient mouse L cells.
To clone the gene for the human Na+/H+ antiporter, we first constructed a stable mouse LTK- cell line (LAP1) lacking Na+/H+ antiport activity. Second, we devised a selective technique based on acid killing that specifically sorts out cells expressing low levels of Na+/H+ antiport activity from a population of antiporter-deficient cells (AP-). LAP1 cells (TK- and AP-) were cotransformed with hum...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1986
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.83.24.9388